ACSI P3 - Y4 Exp Bio Prelim 2024

CANDIDATE NAME CENTRE NUMBER S 2 0 3 1 INDEX NUMBER BIOLOGY 6093/03 Paper 3 Practical Test Candidates answer on the Question Paper. 31 July 2024, Wednesday 1 hour 50 minutes READ THESE INSTRUCTIONS FIRST Write your name and index number on all the work you hand in. Give details of the practical shift and laboratory in the boxes provided. Write in dark blue or black pen. You may use a HB pencil for any diagrams or graphs. Do not use staples, paper clips, glue, correction fluid or highlighters. Answer all questions in the spaces provided on the Question Paper. The use of an approved scientific calculator is expected, where appropriate. You may lose marks if you do not show your working or if you do not use appropriate units. The number of marks is given in brackets [ ] at the end of each question or part question. Shift Laboratory For Examiner’s Use 1 2 3 Total /40 This document consists of 13 printed pages, including this cover page. Anglo-Chinese School (Independent) PRELIMINARY EXAMINATION YEAR FOUR EXPRESS

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3 6093/03/Y4Exp/Prelim/Jul2024 [Turn Over 1 Catalase is an enzyme found in living cells. It catalyses the breakdown of hydrogen peroxide to form water and oxygen. You are going to investigate the effect of catalase concentration on the rate of oxygen production. You have been provided with a celery extract which contains catalase. Read all the instructions but do not carry them out until you have drawn a table for your results in the space provided in 1(a)(ii). If any solution comes into contact with your skin, wash off immediately under cold water. You are advised to wear gloves and eye protection throughout the investigation. Step 1 Label five test-tubes A, B, C, D and E. Put the test-tubes into the test-tube rack. Step 2 Use a measuring cylinder to transfer 10 cm3 of celery extract into test-tube A. Step 3 Use the measuring cylinder to transfer 5 cm3 of celery extract from test -tube A to test-tube B. Use the measuring cylinder to add 5 cm3 of distilled water to test -tube B. Place a stopper in test-tube B and shake it to mix. Step 4 Remove the stopper. Use the measuring cylinder to transfer 5 cm3 of the liquid in test-tube B to test-tube C. Use the measuring cylinder to add 5 cm3 of distilled water to test -tube C. Place a stopper in test-tube C and shake it to mix. Step 5 Remove the stopper. Use the measuring cylinder to transfer 5 cm3 of the liquid in test-tube C to test-tube D. Use the measuring cylinder to add 5 cm3 of distilled water to test -tube D. Place a stopper in test-tube D and shake it to mix. Step 6 Remove the stopper. Use the measuring cylinder to transfer 5 cm3 of the liquid in test-tube D to test-tube E. Use the measuring cylinder to add 5 cm3