2014 HCI H1 Biology Prelims P1 Questions

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2 © Hwa Chong Institution 2014 8875 H1 Biology / JC2 Preliminary Examinations / Paper 1 2 Which statements about plant vacuoles are correct? 1 Vacuoles are surrounded by a single selectively permeable membrane. 2 Vacuoles are the main store of amino acids, especially in actively growing cells. 3 Water passes into vacuoles down a water potential gradient and this helps plant cells remain turgid. 4 The size of the vacuole(s) remains constant in proportion to the size of the cell, regardless of the water content of the cell. A 1 and 2 B 1 and 3 C 1, 3 and 4 D 2, 3 and 4 1 The distribution of membranes in the organelles of three cell types, a prokaryotic cell, a liver cell and an enzyme-secreting cell from the st omach, is determined. For each organelle, the amount of membrane is expressed as a percentage of the total amount of membrane in the cell. Which row of values best represents the membrane distribution in the three cell types? CM : cell membrane rERM : rough endoplasmic reticulum membrane IMM : inner mitochondrial membrane OMM : outer mitochondrial membrane prokaryotic cell liver cell stomach cell CM rERM IMM rERM OMM IMM rERM OMM IMM A 99 0 0 35 7 28 60 16 4 B 99 0 0 35 7 28 60 4 16 C 52 12 36 60 7 28 35 4 16 D 52 12 36 60 28 7 35 4 16

3 © Hwa Chong Institution 2014 8875 H1 Biology / JC2 Preliminary Examinations / Paper 1 3 The diagram shows a circular oligosaccharide molecule. In which other molecule can a similar glycosidic bond be found? A lactose B maltose C sucrose D cellulose 4 The hydrolysis of triglycerides leads to 1 the formation of products which are more soluble in water than triglycerides. 2 the formation of products which are less soluble in water than triglycerides. 3 an increase in pH. 4 a decrease in pH. A 1 and 4 B 2 and 3 C 2 and 4 D 1 and 3

4 © Hwa Chong Institution 2014 8875 H1 Biology / JC2 Preliminary Examinations / Paper 1 5 In an investigation, a solution of enzyme X is placed in a sealed bag made of selectively permeable membrane. The bag is then placed in a beaker of distilled water, and the distilled water is changed several times. Samples of equal volume are then drawn from the bag at regular intervals. To each sample extracted, a fixed amount of substrate was added and the rate of reaction is determined by the number of bubbles formed over 10 minutes . The number of bubbles formed is observed to be inversely proportional to the duration the bag is immersed in distilled water. Which is a valid conclusion that can be made from this investigation? A Some enzyme X molecules have lost their secondary and tertiary structure after some time. B More enzyme X molecules were inhibited by a non-competitive inhibitor present in the solution over time. C Concentration of enzyme X decreases with time. D Enzyme X is moving out of the bag at a slow rate.

5 © Hwa Chong Institution 2014 8875 H1 Biology / JC2 Preliminary Examinations / Paper 1 6 Three batches of cells at the start of G1 phase of the cell cycle were treated with three drugs P, Q and R. Each batch has the same number of cells. Untreated cells are expected to complete interphase within 24 hours. The relative DNA content of the treated cells was measured after 24 hours. The three graphs show the results obtained. Which row correctly identifies the effect of each drug on the dividing cells? What is the effect of each drug on the cells? drug P drug Q drug R A blocks G1 phase blocks S phase blocks G2 phase B blocks G2 phase blocks S phase blocks G1 phase C blocks S phase blocks G1 phase blocks G2 phase D blocks G1 phase blocks G2 phase blocks S phase number of cells 2x drug P drug Q drug R 2x 4x 4x 4x 2x number of cells number of cells relative DNA content relative DNA content relative DNA content

6 © Hwa Chong Institution 2014 8875 H1 Biology / JC2 Preliminary Examinations / Paper 1 Which row matches the stages happening at X and Y indicated? duration of metaphase / min increasing distance at X is due to measurement of X measurement of Y A 35 segregation of sister chromatids distance between each chromatid and the pole distance between chromatids B 18 segregation of sister chromatids distance between chromatids distance between each chromatid and the pole C 35 chromosomes align and segregate distance between chromatids distance between each chromatid and the pole D 18 chromosomes align and segregate distance between each chromatid and the pole distance between chromatids 7 The graph shows information about the movement of chromatids in a cell that has just started metaphase of mitosis. time after start of metaphase / min distance / μm start of metaphase

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8 © Hwa Chong Institution 2014 8875 H1 Biology / JC2 Preliminary Examinations / Paper 1 10 A segment of a polypeptide chain is Arg – Gly – Ser – Phe – Val – Asp – Arg. It is encoded by the following segment of DNA:   strand 1 3