HCI 2023 JC2 9744 H2 Bio Prelim P4 QP (Sharing)

© Hwa Chong Institution 2023 9744 H2 Biology / JC2 Preliminary Examinations / Paper 4 HWA CHONG INSTITUTION JC2 Preliminary Examinations Higher 2 CANDIDATE NAME CT GROUP 22S7 __ CENTRE NUMBER INDEX NUMBER BIOLOGY Paper 4 Practical Candidates answer on the Question Paper. No Additional materials are required. 9744/04 28 August 2023 2 hours 30 minutes INSTRUCTIONS TO CANDIDATES There are two question booklets ( I and II) to this paper. Write your name, CT group, Centre number and index number in the spaces provided at the top of this cover page and on the lines provided at the top of the cover page of Booklet II. Answer all questions in the spaces provided on the question paper. Shift Laboratory INFORMATION FOR CANDIDATES The use of an approved scientific calculator is expected, where appropriate. You may lose marks if you do not show your working or if you do not use appropriate units. The number of marks is given in brackets [ ] at the end of each question or part question. You are reminded of the need for good English and clear presentation in your answers. This document consists of 23 printed pages and 1 blank page. For Examiners' Use 1 / 30 2 / 25 Total / 55

2 © Hwa Chong Institution 2023 9744 H2 Biology / JC2 Preliminary Examinations / Paper 4 QUESTION 1 Part A The process of photosynthesis traps light using a range of pigments bound to chloroplast membranes. The development of these pigments and chloroplasts in young plants is influenced by their growth conditions. In this experiment you are required to investigate the pigments and chloroplasts in two sets of young plants, A and B, grown in different conditions. You must keep the two types of leaves separate throughout the investigation. Observe and make brief notes of any differences between a leaf from plant A and a leaf from plant B. Procedure 1 (To be attempted when you receive the light microscope) 1 Cut the leaves from specimen A into four pieces, each about 3 cm by 3 cm. Set aside two pieces of the leaves from specimen A for Procedure 2. 2 Place two pieces of the leaves from specimen A in a mortar. Add 4 cm3 distilled water and grind with a pestle for 1 minute. 3 Filter the suspension through the muslin cloth provided, onto a Petri dish. 4 Use the Pasteur pipette provided to put a drop of filtrate onto a microscope slide and cover with a coverslip. Observe under ×100 and ×400 magnifications. 5 Repeat steps 1 - 4 using the leaves from specimen B. (a) State two differences between the chloroplasts from specimen A and specimen B. [2] Procedure 2 6 Add 5 cm3 of ethanol into each of the two tubes labelled X provided. Label the tubes A and B respectively with a marker. 7 Put the cut pieces of leaves from specimen A and specimen B on separate clean paper towe