NJC 2023 H2 Biology Prelim P1 QP

©NJC 2023 9744/01/SH2/H2/Biology/Preliminary Examination [Turn over NATIONAL JUNIOR COLLEGE, SINGAPORE Senior High 2 Preliminary Examination Higher 2 CANDIDATE NAME BIOLOGY CLASS 2bi2_____ REGISTRATION NUMBER Biology Paper 1: Multiple Choice Questions Additional Materials: Multiple Choice Answer Sheet 9744/01 15 September 2023 1 hour READ THESE INSTRUCTIONS FIRST Write your name, Biology class and registration number on all the work you hand in. Write in dark blue or black pen on both sides of the paper. You may use a soft pencil for any diagrams, graphs or rough working. Do not use staples, paper clips, highlighters, glue or correction fluid. There are thirty questions on this paper. Answer all questions. For each question there are four possible answers A, B, C and D. Choose the one you consider correct and record your choice in soft pencil on the separate Answer Sheet. Read the instructions on the Answer Sheet very carefully. Each correct answer will score one mark. A mark will not be deducted for a wrong answer. Any rough working should be done in this booklet. The use of an approved scientific calculator is expected, where appropriate. Your five-digit OAS index number is made up of the second and last four digits of your registration number . For example: Registration no. OAS Index no. 2205123 25123 This document consists of 23 printed pages and 1 blank page.

2 ©NJC 2023 9744/01/SH2/H2/Biology/Preliminary Examination 1 Which statement is true? A Centrioles and vesicles can be seen under a light microscope. B Hydrolytic enzymes are mainly found in lysosomes for both animal and plant cells. C Mitochondria share the same 80S ribosome as the prokaryotic cell. D Vesicles from the smooth endoplasmic reticulum carry lipids to the Golgi apparatus for modification. 2 The electron micrograph below shows an organelle. Which of the following statements are true? 1 X is made up of a phospholipid bilayer and it is selectively permeable to substances. 2 X serves to compartmentalise the organelle from the extracellular environment. 3 Y has embedded ATP synthase that synthesises ATP. 4 Y has embedded photosystems that are involved in the absorption of light. 5 Z contains DNA that has histones that help to package DNA present in the organelle. 6 Z contains enzymes that oxidises glucose to pyruvate. A 1, 2 and 3 B 1 and 3 C 2, 4 and 6 D 5 and 6

3 ©NJC 2023 9744/01/SH2/H2/Biology/Preliminary Examination [Turn over 3 The diagram shows two isomers of a hexose labelled I and II. Four possible bonding positions are labelled d, e, f, g and h on one isomer, and s, t, u, v and w on another isomer. isomer I isomer II Which isomer and bonding positions are involved in the formation of glycogen? isomer bonding positions A isomer I d-g and d-h B isomer I e-h and d-h C isomer II s-v and s-w D isomer II t-w and s-w 4 Which of the following statement(s) is / are true? 1 Formation of a peptide bond involves a reaction between an amino group and a carboxyl group. 2 In a neutral solution, most amino acids exists as positively charged compounds. 3 The secondary structure of a polypeptide chain is formed as a result of hydrogen bonds formed between the R-groups. 4 The primary structure of a protein will most likely be preserved when a protein is denatured. A 1 and 4 only B 1 only C 2 only D 3 and 4 only

4 ©NJC 2023 9744/01/SH2/H2/Biology/Preliminary Examination 5 The diagram illustrates the types of membrane phospholipids found in the intact erythrocytes (red blood cells). Which of the following statements can be concluded from the graph? 1 76% of the total membrane phospholipids contain choline and 20% contain ethanolamine. 2 24% of PC and 80% of PE are confined to the inner surface of the erythrocytes. 3 Most PC is confined to the outer surface of the erythrocytes while most of the PE are confined to the inner surface of the erythrocytes. 4 Membranes in most cell types can be concluded to be asymmetric. A 1 and 2 only B 1 and 3 only C 2 and 3 only D 2 and 4 only

5 ©NJC 2023 9744/01/SH2/H2/Biology/Preliminary Examination [Turn over 6 The diagram illustrates various features of embryonic stem cells and specialised cells. Which row correctly describes the features of embryonic stem cells and specialised cells? embryonic stem cells specialised cells A Embryonic stem cells are multipotent and are capable of differentiating into limited range of cell types. The blood cells are genetically different from the embryonic stem cells because different genes are expressed. B Embryonic stem cells are pluripotent and are capable of differentiating into many different cell types. The pancreatic cells are genetically identical to the embryonic stem cells but have shorter telomeres. C Embryonic stem cells are totipotent and are capable of differentiating into many different cell types. The pancreatic cells are genetically identical to the embryonic stem cells but with a different set of genes expressed. D Embryonic stem cells display greater potential to differentiate when grown in culture than when in blastocyst. The muscle cells are genetically different from the embryonic stem cells but have shorter telomeres.

6 ©NJC 2023 9744/01/SH2/H2/Biology/Preliminary Examination 7 How many different polypeptides, each consisting of r amino acids, can be made if there are n different amino acids? A n B nr C nr D rn 8 CFTR is a transmembrane regulator protein. It is made up of 1480 amino acids. People with cystic fibrosis produce a defective CFTR protein which is missing one amino acid from its structure. A normal CFTR protein molecule has sugar molecules attached to it which make it functional. Which of the following statements are true? 1 4440 is the number of bases on the template DNA which code for the amino acid sequence of a normal CFTR protein. 2 Cystic fibrosis is due to a chromosomal mutation known as a deletion. 3 The defective CFTR is not functional as the mutation in the DNA resulted in changes in its tertiary structure. 4 The functional CFTR is a protein that undergone glycosylation. A 1, 3 and 4 only B 1 and 3 only C 1 and 4 only D 2, 3 and 4 only

7 ©NJC 2023 9744/01/SH2/H2/Biology/Preliminary Examination [Turn over 9 The diagram shows a T4 bacteriophage attaching to its host cell. Which combination correctly identifies the constituents of the lettered components? V W X Y A amino acids DNA nucleotides amino acids peptidoglycan B amino acids RNA nucleotides lipids lipids C lipids DNA nucleotides amino acids lipids D lipids RNA nucleotides lipids peptidoglycan W V Y

8 ©NJC 2023 9744/01/SH2/H2/Biology/Preliminary Examination 10 An experiment was carried out to investigate the effect of bacteriophages and antibiotics on Escherichia coli. Two strains of E. coli were used where one of the strains was resistant to antibiotics. Three discs of filter papers were soaked with either temperate phages, virulent phages or antibiotics and placed on an agar plate spread with one strain of E. coli. The plate was then incubated at 37°C for 10 hours and rings of clear zones were observed. The diagram below shows the results. The same experiment was repeated on a second agar plate containing the other strain of E. coli and there was only one ring of clear zone around filter paper Y. Which explanation could be deduced from the experiment? A Filter paper X was soaked in antibiotics but the strain of E. coli used in the second plate was resistant to antibiotics, resulting in no clear zone around filter paper X. B Filter paper X was soaked with phages that did not initiate an immediate lysis of E. coli cells, res