2026 HCI 9477 H2 Biology Prelim P4 QP

HWA CHONG INSTITUTION JC2 Preliminary Examinations Higher 2 CANDIDATE NAME CT GROUP 25S7 __ CENTRE NUMBER S INDEX NUMBER BIOLOGY Paper 4 Practical Candidates answer on the Question Paper. Additional Materials: As listed in the Confidential Instructions. 9477/04 31 August 2026 2 hours 30 minutes READ THESE INSTRUCTIONS FIRST There are three question booklets ( I, II and III ) to this paper. Write your name , CT group , Centre number and index number in the spaces provided at the top of this cover page and on the lines provided at the top of the cover page of Booklets II and III . Give details of the practical shift and laboratory, where appropriate, in the boxes provided. Write in dark blue or black pen. You may use an HB pencil for any diagrams or graphs. Do not use staples, paper clips, glue or correction fluid. Answer all questions in the spaces provided on the Question Paper. The use of an approved scientific calculator is expected, where appropriate. You may lose marks if you do not show your working or if you do not use appropriate units. At the end of the examination, fasten all your work securely together. The number of marks is given in brackets [ ] at the end of each question or part question. This document consists of 20 printed pages. © Hwa Chong Institution 2026 9477 H2 Biology / JC2 Preliminary Examinations / Paper 4 Shift Laboratory For Examiners' Use 1 / 21 2 / 14 3 / 15 Total / 50

2 Answer all questions. QUESTION 1 Dialysis tubing is a partially permeable membrane. Glucose molecules can diffuse through the dialysis tubing. You are required to investigate the diffusion of glucose across dialysis tubing. You are provided with the materials shown in Table 1.1. Table 1.1 labelled contents hazard volume / cm 3 G 20.0% glucose solution low 50 W distilled water low 200 B Benedict’s solution harmful irritant 40 D length of dialysis tubing in distilled water low - If any solution comes into contact with your skin, wash off immediately with cold water. It is recommended that you wear suitable eye protection. You will need to: ● put glucose solution into dialysis tubing surrounded by water ● take samples of the water surrounding the dialysis tubing ● test for the presence of glucose in each sample of water. © Hwa Chong Institution 2026 9477 H2 Biology / JC2 Preliminary Examinations / Paper 4

3 Carry out step 1 to step 11 . 1 Draw a mark 8 cm from the top of a large test-tube, as shown in Fig. 1.1. Fig. 1.1 2 Remove the dialysis tubing from plastic vial D . Tie a knot in the dialysis tubing as close as possible to one end, so that the end is sealed. 3 The whole length of the dialysis tubing needs to be separated to allow the tubing to be filled with solution. To do this, rub the whole length gently between your finger and thumb. 4 Put 10 cm 3 of 20.0% glucose solution, G , into the open end of the dialysis tubing. 5 Rinse the outside of the dialysis tubing by dipping it in the water in plastic vial D . 6 Put the dialysis tubing containing G into the large test-tube and keep it in position using an elastic band as shown in Fig. 1.2. Fig. 1.2 © Hwa Chong Institution 2026 9477 H2 Biology / JC2 Preliminary Examinations / Paper 4

4 7 Put distilled water into the large test-tube so that the top of the water is above the level of the glucose solution in the dialysis tubing. 8 Start timing. You are required to take samples of the water surrounding the dialysis tubing every 5 minutes for 15 minutes. You will collect three samples. 9 Label three test-tubes with the times the samples of water will be taken ( 5 , 10 and 15 ). 10 After 5 minutes (step 8 ), put a 1 cm 3 syringe into the water surrounding the dialysis tubing so that the end of the syringe is level with the mark on the test-tube. Remove 1 cm 3 from the water surrounding the dialysis tubing and put this into the test-tube labelled 5 . Repeat this action to remove another 1 cm 3 from the water surrounding the dialysis tubing and put this into the test-tube labelled 5 . The test-tube labelled 5 will now contain a 2 cm 3 sample. Do not stop timing. 11 Repeat step 10 at 10 minutes and at 15 minutes using the appropriately labelled test-tubes. You need to carry out a dilution of the 20.0% glucose solution, G , to make a 1.0% glucose solution. You will need 20 cm 3 of this 1.0% glucose solution. Table 1.2 volume of 20.0% glucose solution, G / cm 3 volume of distilled water, W / cm 3 1 19 12 Prepare the 1.0% glucose solution as shown in Table 1.2. You will need to carry out a serial dilution of the 1.0% glucose solution you have prepared in step 12 as shown in Fig. 1.3. You must reduce the concentration by half between each successive dilution. You will need to prepare four concentrations of glucose solution in addition to the 1.0% glucose solution from step 12 . After the serial dilution is completed, you will need to have 10 cm 3 of each concentration available to use. © Hwa Chong Institution 2026 9477 H2 Biology / JC2 Preliminary Examinations / Paper 4

5 (a)(i) Complete Fig. 1.3 to show how you will prepare your serial dilution. Each beaker should have: ● a labelled arrow to show the volume of glucose solution transferred ● a labelled arrow to show the volume of distilled water, W , added ● a label under the beaker to show the concentration of glucose solution. © Hwa Chong Institution 2026 9477 H2 Biology / JC2 Preliminary Examinations / Paper 4

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7 13 Set up a boiling water-bath ready for step 18 and step 22 . 14 Prepare the concentrations of glucose solution as shown in Fig. 1.3. 15 Label 5 te