2016 VJC H2 Biology Prelims Paper 3 Questions

1 VICTORIA JUNIOR COLLEGE BIOLOGY DEPARTMENT JC2 PRELIMINARY EXAMINATIONS 2016 Higher 2 VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE VICTORIA JUNIOR COLLEGE CANDIDATE NAME CLASS INDEX NUMBER BIOLOGY 9648/03 Paper 3 19 September 2016 Additional Materials: Answer Booklet/ Paper 2 Hours READ THESE INSTRUCTIONS FIRST Write your CT GP/ INDEX NO. and name on all the work you hand in. Write in dark blue or blue pen. You may use a soft pencil for any diagrams, graphs or rough working. Do not use any staples, paper clips, highlighters, glue or correction fluid. Answer all questions. At the end of the examination, fasten all your work securely together. The intended number of marks is given in brackets [ ] at the end of each question. This document consists of 16 printed pages. 1 5 S For Examiner’s Use Section A 1 2 3 Planning Question 5 Total

2 Answer all questions Question 1 (a) The bacterial plasmid, pBR322, was used as a ve ctor for Gene X as shown in Fig. 1.1 below. Gene X was inserted in the BamHI restriction site. pBR322 also contain the EcoRI and HpaI restriction sites. The target sites for these restriction enzymes are shown in the table below. The line s drawn in each sequence show where the enzyme cuts the DNA molecule.   (i) With reference to Fig. 1.1, explain how two properties of plasmid pBR322 allow it to be used as a vector. ………………………………………………………………………………………………….. ………………………………………………………………………………………………….. ………………………………………………………………………………………………….. …………………………………………………………………………………………………. [2] EcoRI site EcoRI site

3 (ii) Outline the steps taken to produce the recombinant plasmid shown in Fig. 1.1. ………………………………………………………………………………………………….. ………………………………………………………………………………………………….. ………………………………………………………………………………………………….. …………………………………………………………………………………………………. [2] (iii) Explain the disadvantage that would arise if gene X was to be inserted into the HpaI restriction site instead of the BamHI site. ………………………………………………………………………………………………….. ………………………………………………………………………………………………….. ………………………………………………………………………………………………….. ……………………