2015 HCI H2 Biology Prelims Paper 3 Questions

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2 © Hwa Chong Institution 2015 9648 H2 Biology / Preliminary Exams / Paper 3 BOOKLET I STRUCTURED QUESTIONS QUESTION 1 Restriction enzymes are used in recombinant DNA technology (RDT). Fig. 1.1 shows the palindromic recognition sequence of EcoRI, a restriction enzyme. 5’  N  N  G  A  A  T  T  C  N  N  3’ 3’  N  N  C  T  T  A  A  G  N  N  5’ Fig. 1.1 (a) Explain what is meant by the term palindromic. [2] The bacterial plasmid, pUC, is a useful cloning vector in RDT. A researcher is investigating the use of two restriction enzymes, EcoRI and BamHI, to clone gene X into pUC. Fig. 1.2 shows the results when pUC and recombinant pUC are treated with either EcoRI, BamHI or both. pUC / kb recombinant pUC / kb EcoRI BamHI both EcoRI BamHI both 7.0 4.0 4.0 7.0 4.0 4.0 3.0 2.0 1.0 2.8 2.0 1.0 1.2 1.0 0.8 0.2 Fig. 1.2

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