2016 RVHS H2 Biology Prelims Paper 1 Questions

River Valley High School Page 1 of 25 Year 6 H2 Biology 9648 Paper 1 2016 Preliminary Examination II RIVER VALLEY HIGH SCHOOL YEAR 6 PRELIMINARY EXAMINATION II H2 BIOLOGY 9648 PAPER 1 26 SEP 2016 1 HOUR 15 MIN CANDIDATE NAME CENTRE NUMBER S INDEX NUMBER READ THESE INSTRUCTIONS FIRST Write in soft pencil. Do not use staples, paper clips, highlighters, glue or correction fluid. Write your name, centre and index number on the Answer Sheet in the spaces provided. DO NOT WRITE IN ANY BARCODES. There are forty questions in this paper. Answer all questions. For each question, there are four possible answers, A, B, C and D. Choose the one you consider correct and record your choice in soft pencil on the separate Answer Sheet. Read the instructions on the Answer Sheet very carefully. Each correct answer will score one mark. A mark will not be deducted for a wrong answer. Any rough working should be done in this booklet. The use of an approved scientific calculator is expected, where appropriate. ____________________________________________________________________________ This Question Paper consists of 25 printed pages.

River Valley High School Page 2 of 25 Year 6 H2 Biology 9648 Paper 1 2016 Preliminary Examination II For each question, there are four possible answers, A, B, C and D. Choose the one you consider correct and record your choice in soft pencil on the separate Answer Sheet. 1 A sample of yeast cells were grown in a culture with radioactive amino acids. At various times, samples of the cells were taken and the amount of radioactivity in different organelles was measured. The results are shown in the table below. Time after radioactive amino acids were added to the solution/ minute Amount of radioactivity present/arbitrary units P Q R 10 21 120 6 20 42 68 6 40 86 39 8 60 76 28 15 90 50 27 28 120 38 26 56 Which of the following best describes the identities of organelles P, Q and R? P Q R A Golgi apparatus Rough endoplasmic reticulum Secretory vesicles B Rough endoplasmic reticulum Smooth endoplasmic reticulum Golgi apparatus C Rough endoplasmic reticulum Secretory vesicles Golgi apparatus D Smooth endoplasmic reticulum Golgi apparatus Secretory vesicles

River Valley High School Page 3 of 25 Year 6 H2 Biology 9648 Paper 1 2016 Preliminary Examination II 2 Three unknown specimens were tested to determine the identity of biomolecule(s) present. The results are shown in the table below. biuret solution benedict’s solution and heated dilute hydrochloric acid, benedict’s solution and heated iodine in potassium iodide solution Specimen 1 purple blue brick-red brown Specimen 2 purple blue blue blue-black Specimen 3 blue brick-red brick-red brown Using the results shown, which of the following can be concluded? 1 When treated with amylase, Specimen 2 will yield the same result as Specimen 1. 2 No lipids are present in Specimens 1, 2 and 3. 3 Sucrose is present in Specimen 3. A 2 only B 1 and 2 C All of the above D None of the above 3 The diagram shows the chemical structure of a polypeptide chain. The polypeptide chain can be broken down by proteases to yield amino acids. Which of the following combination is correct? type of reaction number of bonds broken number of amino acids A hydrolysis 5 6 B hydrolysis 4 5 C condensation 6 7 D condensation 7 6

River Valley High School Page 4 of 25 Year 6 H2 Biology 9648 Paper 1 2016 Preliminary Examination II 4 In the production of isoleucine from threonine, the end product acts as an inhibitor of the first enzyme of the pathway. The pathway is shown below. Which of the following graphs shows the concentration of intermediate Y when threonine is supplied in excess? A B C D 5 The diagram below shows a stage of mitosis in a cell. The amount of DNA present is 12 picograms (pg). Which row correctly identifies the number of DNA molecules and amount of DNA in each nucleus at different stages of nuclear division? telophase of mitosis telophase II of meiosis number of DNA molecules amount of DNA / pg number of DNA molecules amount of DNA / pg A 12 6 6 3 B 24 12 12 6 C 12 12 6 3 D 24 6 12 6

River Valley High School Page 5 of 25 Year 6 H2 Biology 9648 Paper 1 2016 Preliminary Examination II 6 The table below shows the events that occur at different stages of meiosis. Which row correctly shows the correct events for the respective stage? Interphase II Prophase I Metaphase I Anaphase II A replication of DNA condensation of chromosomes alignment of chromosomes at the equator separation of homologous chromosomes B replication of DNA pairing of bivalents alignment of bivalents at the equator separation of sister chromatids C intense protein synthesis crossing over alignment of bivalents at the equator separation of sister chromatids D replication of organelles pairing of bivalents alignment of chromosomes at the equator separation of homologous chromosomes 7 An unknown organism has a linear double -stranded DNA genome like that in a eukaryote. When its DNA replication was examined , i t was revealed that although the process is semi -conservative, no Okazaki fragments were observed in the multiple replication forks. In addit ion, the end -replication problem of shorten ed daughter strands was not observed. Which statement correctly explains this phenomenon? A The organism’s DNA is antiparallel. B DNA replication only starts at the 3’ end of each template strand. C DNA polymerases synthesise DNA in both 5’ to 3’ and 3’ to 5’ direction. D DNA ligases are not involved in the DNA replication process.

River Valley High School Page 6 of 25 Year 6 H2 Biology 9648 Paper 1 2016 Preliminary Examination II 8 The electron micrograph shows protein synthesis in a yeast cell. Which statements about processes occurring in this cell are correct? 1 Transcription occurs from left to right along template DNA. 2 S is used as template during translation. 3 Transcription and translation occur simultaneously in the cell. 4 Many RNA polymerases are transcribing R. A 1 and 3 only B 2 and 4 only C 1, 2 and 4 D All of the above 9 Which statements about tRNA are correct? 1 There is a binding site for the attachment of a specific amino acid, as well as a different binding site for the attachment to the ribosome, in order to allow translation to occur. 2 There is a ribose -phosphate backbone with strong covalent phosphoester bonds and areas within the polynucleotide chain where base -pairing by hydrogen bonding occurs. 3 There is a section known as an anticodon that contai ns the same triplet of bases as the triplet of DNA bases that has been transcribed to produce the mRNA codon. 4 There is a specific enzyme to load each tRNA with its respective amino acid. A 1 and 2 only B 2 and 3 only C 1, 2 and 3 D 1, 2 and 4 10 A segment of an mRNA sequence bearing a point mutation is shown. What is the sequence of the corresponding DNA coding strand prior to the mutation? 5’-ACCGUAGCAGCU-3’ A 5’-AGCTGCTACGGT-3’ B 5’-ACCGTAGCAGCT-3’ C 5’-ACCGGAGCAGCT-3’ D 5’-AGCTGCTCCGGT-3’ R S

River Valley High School Page 7 of 25 Year 6 H2 Biology 9648 Paper 1 2016 Preliminary Examination II 11 In 2015, Ebola Virus Disease outbreak in West Africa is the largest outbreak of the disease since the Ebola virus was first identified. Ebola virus is an animal virus and has structural components similar to that of influenza virus and human immunodeficien cy virus. The structure of the Ebola virus is shown below. Which of the following correctly identifies the function of the labelled structures? bind to specific receptor on human cell surface membrane contai