2025 RVHS H2 Biology Prelims Paper 4_QP

RIVER VALLEY HIGH SCHOOL JC 2 PRELIMINARY EXAMINATION CANDIDATE NAME CENTRE NUMBER S CLASS 24J INDEX NUMBER H2 BIOLOGY 9744/04 Paper 4 Practical 28 Aug 2025 Candidates answer on the Question Paper. 2 hours 30 minutes Additional Materials: As listed in the Confidential Instructions. READ THESE INSTRUCTIONS FIRST Write your Centre number, index number, class and name on all the work you hand in. Give details of the practical shift and laboratory, where appropriate, in the boxes provided. Write in dark blue or black pen. You may use an HB pencil for any diagrams or graphs. Do not use staples, paper clips, glue or correction fluid. DO NOT WRITE ON ANY BARCODES. Answer all questions in the spaces provided on the Question Paper. The use of an approved scientific calculator is expected, where appropriate. You may lose marks if you do not show your working or if you do not use appropriate units. At the end of the examination, fasten all your work securely together. The number of marks is given in brackets [ ] at the end of each question or part question. Shift Laboratory For Examiner’s Use 1 2 Total This document consists of 16 printed pages and 2 blank pages.

2 River Valley High School 2025 JC2 Preliminary Examination H2 Biology 9744/04 Answer all questions. 1 A farmer claimed that the carrots he grew contained a high sugar concentration and that the sugars were more effectively taken up by the gut as compared to standard carrot breeds. Carrots generally contain different types of sugars, with sucrose and simple sugars being the most abundant. In this experiment, you will investigate the validity of his claims. You are required to: • perform serial dilution to obtain different concentrations of S • estimate the sugar concentration in X • determine if sugars in the farmer-grown carrots are more effectively taken up. You are provided with: • 100 cm 3 of 1.0 mol dm -3 sugar solution obtained from standard carrot breed s, in a beaker labelled S • 50 cm3 of farmer-grown carrot extract, in a beaker labelled X • Visking tubing, in a beaker of distilled water labelled D • distilled water, in a beaker labelled W. Before starting the investigation, read through steps 1-24 and prepare a table in (c).

3 River Valley High School 2025 JC2 Preliminary Examination H2 Biology 9744/04 Proceed as follows. 1 You are required to make a proportional dilution of 1.0 mol dm -3 sugar solution S, to reduce its concentration by five-fold between each successive dilution. After the serial dilution is completed, you will need to have at least 40 cm3 of each concentration available for use. (a) Complete Fig. 1.1 to show how you will dilute S. For each specimen tube: • state, under the tube, the volume and concentration of the solution S in the tube that will be available for use in the investigation, after the