2026 HCI 9477 H2 Bio Prelim P1 QP

HWA CHONG INSTITUTION JC2 Preliminary Examinations Higher 2 CANDIDATE NAME CT GROUP 25S7 __ CENTRE NUMBER S INDEX NUMBER BIOLOGY Paper 1 Multiple Choice Additional Materials: Multiple Choice Answer Sheet. 9477/01 22 September 2026 1 hour READ THESE INSTRUCTIONS FIRST Write your name , CT group, Centre number and index number in the spaces provided at the top of this cover page. Fill in your particulars on the Multiple Choice Answer Sheet. Write and shade your Student ID. Your student ID is xxxxxxx, where your HCI email is xxxxxxx@student.hci.edu.sg Write in soft pencil. Do not use staples, paper clips, glue or correction fluid. There are thirty questions on this paper. Answer all questions. For each question, there are four possible answers, A , B , C and D . Choose the one you consider correct and record your choice in soft pencil on the separate Answer Sheet. At the end of the paper, you are to submit only the Answer Sheet. INFORMATION FOR CANDIDATES Each correct answer will score one mark. A mark will not be deducted for a wrong answer. Any rough working should be done in this booklet. The use of an approved scientific calculator is expected, where appropriate. © Hwa Chong Institution 2026 9477 H2 Biology / JC2 Preliminary Examinations / Paper 1

2 This document consists of 23 printed pages and 1 blank page. © Hwa Chong Institution 2026 9477 H2 Biology / JC2 Preliminary Examinations / Paper 1

3 1 The diagram shows an electron micrograph of a cell. Which row correctly describes structures P, Q and R? P Q R A provides large surface area for attachment of ribosomes modification of transcripts active condensation of chromatin B transport of proteins to Golgi apparatus formation of ATP from light energy active transcription of genes C synthesis and processing of membrane proteins sequestering of protons within a specialised compartment active transcription of genes D protects proteins from degradation by cytoplasmic enzymes allows the oxidation of pyruvate active replication of DNA © Hwa Chong Institution 2026 9477 H2 Biology / JC2 Preliminary Examinations / Paper 1

4 2 The table compares some features of W, X, Y and Z. chlorophyll DNA RNA histones W ✔ ✔ ✔ ✔ X

✔ ✔

Y

✔ ✔

Z

✔

key ✔ = present

= not present Which row correctly identifies W, X, Y and Z? W X Y Z A plant cell virus mitochondrion bacterium B plant cell mitochondrion bacterium virus C mitochondrion virus bacterium plant cell D virus bacterium mitochondrion plant cell 3 Which row correctly pairs a core feature of cell theory with a specific viral characteristic that challenges its validity? feature of cell theory viral characteristic that challenges it A the cell is the smallest unit of life viruses are encapsulated by a protein capsid instead of a cellulose or peptidoglycan cell wall © Hwa Chong Institution 2026 9477 H2 Biology / JC2 Preliminary Examinations / Paper 1

5 B all living things are composed of one or more cells viruses exhibit biological replication and evolution but are entirely acellular C all cells arise from pre-existing cells viruses can undergo rapid genetic mutations and recombination during replication D the cell is the functional unit of life viruses possess specific surface glycoproteins to recognise and bind to host cell receptors © Hwa Chong Institution 2026 9477 H2 Biology / JC2 Preliminary Examinations / Paper 1

6 4 The diagram shows representations of the structures of four polysaccharides. Which statements are correct? 1 Amylopectin and glycogen are highly branched. 2 Amylose and cellulose contain α- glucose monomers joined by 1-4 glycosidic bonds. 3 Glycogen contains the highest proportion of 1-6 glycosidic bonds. 4 All the four polysaccharides are used for energy storage. A 1 and 2 only B 1 and 3 only C 2 and 3 only D 1, 2, 3 and 4 © Hwa Chong Institution 2026 9477 H2 Biology / JC2 Preliminary Examinations / Paper 1

7 © Hwa Chong Institution 2026 9477 H2 Biology / JC2 Preliminary Examinations / Paper 1

8 5 The diagram shows a triglyceride molecule. Which statement correctly describes the structure and function of this triglyceride? A The glycerol head forms hydrogen bonds with water in cytoplasm to produce oil droplets. B The hydrophobic fatty acids allow only small molecules to pass through the cell surface membrane. C It packs closely with identical triglyceride molecules to form energy reserves in lysosomes. D It has long hydrocarbon chains that release high energy output per unit mass when oxidised. 6 A method called freeze-fracture can be used to study the structure of cell membranes. The membrane is frozen and then split down the middle, separating the two layers of phospholipids from each other. Any proteins contained within one layer remain in that layer. Proteins which span the membrane can be found in either layer after freeze-fracture, depending on the properties of the protein. Which statement about the results of freeze-fracture studies are correct? 1 It provides evidence for the bilayer nature of membranes. 2 It provides evidence for the arrangement of proteins. 3 It shows that the proteins in the membrane do not contribute to its strength. A 1 only B 1 and 2 only C 2 and 3 only D 1, 2 and 3 © Hwa Chong Institution 2026 9477 H2 Biology / JC2 Preliminary Examinations / Paper 1

9 © Hwa Chong Institution 2026 9477 H2 Biology / JC2 Preliminary Examinations / Paper 1

10 7 The graph shows how the rate of a reaction changes with substrate concentration in the