RVHS 10. Control of Gene Expression - Eukaryotes 9477

River Valley High School 2025 JC1 H2 Biology Lecture Topic 10: Control of Gene Expression - Eukaryotes 1 River Valley High School 2025 JC1 H2 Biology Lecture Topic 10: Control of Gene Expression - Eukaryotes Name: ( ) Class: 25J _____ Date: References Title Authors Biology (8th Edition) Campbell and Reece Biology: An Australian Focus (3rd Edition) Knox, Ladiges, Evans and Saint Molecular Cell Biology (6th Edition) Lodish, Berk, Kaiser, Krieger, Scott, Bretscher, Ploegh and Matsudaira Molecular Biology of the Cell (5th Edition) Alberts, Johnson, Lewis, Raff, Roberts and Walter Principles of Genetics (3rd Edition) Snustad and Simmons Websites URL Description http://highered.mcgraw- hill.com/sites/9834092339/student_view0/chapter10/chr omatin_remodeling.html transcriptional activation and regulation. https://highered.mheducation.com/sites/9834092339/stu dent_view0/chapter15/processing_of_gene_information __prokaryotes_vs__eukaryotes.html basic differences between prokaryotic and eukaryotic gene expression. https://highered.mheducation.com/sites/9834092339/stu dent_view0/chapter15/rna_splicing.html summary of the splicing process.

River Valley High School 2025 JC1 H2 Biology Lecture Topic 10: Control of Gene Expression - Eukaryotes 2 H2 Biology Syllabus 9477 (2025) Candidates should be able to use the knowledge gained in the following section(s) in new situations or to solve related problems. Related Topics Content Organisation of Genomes Control of Gene Expression - Prokaryotes The structure of eukaryotic chromatin Operons Learning Outcomes 2C. Control of Gene Expression b. Explain how differential (i.e. spatial and temporal) gene expression in eukaryotes can be regulated at different levels: i. Chromatin level (histone modification and DNA methylation); ii. Transcriptional level (control elements, such as promoters, silencers and enhancers, and proteins, such as transcription factors and repressors); iii. Post-transcriptional level (processing of pre -mRNA in terms of splicing, polyadenylation and 5’ capping); iv. Translational level (half-life of RNA, 5’ capping, initiation of translation); and v. Post-translational level (biochemical modification and protein degradation). c. Describe the principles and procedures of these molecular techniques: i. Polymerase chain reaction (including its advantages and limitations); ii. Gel electrophoresis; and iii. Southern blotting and nucleic acid hybridisation. Lecture Outline I. Control of Gene Expression A. Chromatin-level Control A1. Availability of genes for transcription A1.1. Histone modification A1.2. DNA methylation B. Transcriptional Control B1. Initiation of transcription B1.1. Control elements B1.2. Transcription factors C. Post-transcriptional Control via P