Tut 1 - Cell Ultrastructure (Student)

St Andrew’s Junior College Cell Ultrastructure 2021 H2 Biology 1 Full Name: Civics group: 21S Index no.: Date: Core Idea 1: The Cell and Biomolecules of Life Tutorial 1 Cell Ultrastructure MCQ ANSWERS 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 1 Which size ranges can be viewed using a light microscope? A 4 only B 1 and 2 only C 2 and 3 only D 3 and 4 only 2 The diagram shows a chloroplast drawn from an electronmicrograph. The length of the chloroplast from X to Y is 5000 nm. What is the magnification of the drawing of the chloroplast? A x100 B X1000 C X10 000 D X100 000

St Andrew’s Junior College Cell Ultrastructure 2021 H2 Biology 2 3 The electronmicrograph is of a chloroplast. The length of the chloroplast image along the line shown is 90 mm. T he magnification of the organelle is x300,000. What is the actual length of the chloroplast? A 0.3 µm B 3.0 µm C 30 µm D 300 µm 4 You are told that the cells on a microscope slide are plant, animal, or bacterial. You look at them through a microscope and see cell walls and membrane-bound organelles. You conclude that the cells are A Bacteria cells B Plant cells C Bacteria and plant cells D Bacteria, plant, and animal cells

St Andrew’s Junior College Cell Ultrastructure 2021 H2 Biology 3 5 Which features enable an organism to be identified as a prokaryote? 1 cell wall 2 circular DNA 3 nucleus 4 ribosomes A 2 only B 3 only C 1 and 4 only D 2 and 4 only 6 A scientist carried out an experiment to separate the organelles in an animal cell by mass. The scientist mixed the cells with a buffer solution which had the same water potential as the cells. He then broke the cells open with a blender to release the organelles. The extracted mixture was filtered and then spun in a centrifuge at a high speed to separate the heaviest organelle. This sank to the bottom, forming a solid pellet, 1. The liquid above pellet 1 was poured into a clean centrifuge tube and spun in the centrifuge at a higher speed to separate the next heaviest organelle. This organelle sank to the bottom, forming a solid pellet, 2. He repeated this procedure twice more to obtain pellet 3 and pellet 4, each containing a single organelle. What is the function of the organelle extracted in pellet 2? A digestion of old organelles B production of ATP C production of mRNA D synthesis of protein

St Andrew’s Junior College Cell Ultrastructure 2021 H2 Biology 4 In-class activity Arrange the following organelles in the order that they will be separated via cell fractionation: Endoplasmic reticulum, chloroplasts, nucleus, ribosomes, lysosomes, mitochondria 1 What do 70S and 80S mean? S = sedimentation coefficient of a particle (its behaviour during a sedimentation process – centrifugation) What is centrifugation? Application o