2015 RI H2 Biology Prelims Paper 3 Answers

© RI 2015 Preliminary Examination 9648/03 [Turn over RAFFLES INSTITUTION 2015 Year 6 Preliminary Examination Higher 2 BIOLOGY 9648/03 Paper 3 22nd SEPTEMBER 2015 2 hours Additional materials: Answer Sheet READ THESE INSTRUCTIONS FIRST Write your index number, CT group & name on all the work you hand in. Write in dark blue or black pen on both sides of the paper. You may use a soft pencil for any diagrams, graphs or rough working. Do not use staples, paper clips, highlighters, glue or correction fluid. Sections A and B Answer all questions. At the end of the examination, hand in your essay SEPARATELY. The number of marks is given in brackets [ ] at the end of each question or part question. This document consists of 13 printed pages. CIVICS GROUP CANDIDATE NAME INDEX NUMBER 5 S 0 3 1 For Examiner’s Use Section A 1 /12 2 /13 3 /15 4 /12 Section B 5 /20 Total /72 Raffles Institution Internal Examination

2 © RI 2015 Preliminary Examination 9648/03 For Examiner’s Use Section A Answer all the questions in this section. 1 Plasmid X is used to clone a gene of interest. Fig. 1.1 shows a diagram of plasmid X. The restriction sites of four restriction enzymes are shown in Table 1.1. Fig. 1.1 Restriction Enzyme Restriction Sites AaaI 5' ---T GTACA--- 3' 3' ---ACATG T--- 5' Acc65I 5' ---G GTACC--- 3' 3' ---CCATG G--- 5' HaeIII 5' ---GG CC--- 3' 3' ---CC GG--- 5' XmaI 5' ---C CCGGG--- 3' 3' ---GGGCC C--- 5' Table 1.1 (a) (i) With reference to the information in Table 1.1 , describe the characteristics of a restriction site. [2] 1. Restriction site is a specific DNA sequence consists of 4 to 6 base pairs ; 2. which is palindromic* where reading from 5’ to 3’ (or 3’ to 5’) of each of the strands yield the same sequence

3 © RI 2015 Preliminary Examination 9648/03 [Turn over For Examiner’s Use (ii) The restriction enzyme PuvII recognises a 6 base pair sequence o n double stranded DNA. The first three bases of one strand are given, complete the restriction site for PuvII. [1] 5’ C A G _ _ _ 3’