2026 ACJC H2 Biology Prelim P2 QP

This document consists of 24 printed pages and 2 blank pages. [Turn over Anglo-Chinese Junior College JC2 Biology Preliminary Examination Higher 2 CANDIDATE NAME FORM CLASS TUTORIAL CLASS INDEX NUMBER BIOLOGY Paper 2 Structured Questions Candidates answer on the Question Paper. No Additional Materials are required. 9477/02 28 August 2026 2 hours READ THESE INSTRUCTIONS FIRST Write your Name, Class and Index number in the spaces at the top of this page. Write in dark blue or black pen. You may use an HB pencil for any diagrams or graphs. Do not use staples, paper clips, glue or correction fluid. Answer all questions in the spaces provided on the Question Paper. The use of an approved scientific calculator is expected, where appropriate. You may lose marks if you do not show your working or if you do not use appropriate units. The number of marks is given in brackets [ ] at the end of each question or part question. For Examiners’ use only 1 / 11 2 / 11 3 / 8 4 / 8 5 / 11 6 / 9 7 / 11 8 / 10 9 / 5 10 / 6 Total / 90

2 Anglo-Chinese Junior College 2026 J2 H2 9477 Paper 2 Preliminary Examination For Examiner Use Answer all questions. 1 Figure 1.1 is an electron micrograph of part of a chloroplast of a maize leaf cell. Figure 1.1 (a) (i) Table 1.1 shows some substrates and products involved in photosynthesis. Use letter A or letter B from Figure 1.1 to complete Table 1.1 to show the location where the substrates or products are used or produced. Table 1.1 substrate or product location (A or B) carbon dioxide used reduced NADP used ATP produced hexose produced …………………………………………………………………………………………........... . [2]

3 [Turn over Anglo-Chinese Junior College 2026 J2 H2 9477 Paper 2 Preliminary Examination For Examiner Use (ii) Chloroplasts contain numerous structures labelled A that are stacked together . Explain how this increases the efficiency of photosynthesis. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………............ [3] (b) Define the absorption and action spect ra of photosynthetic pigments and describe their relationship. …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... ……………………………………………………………………………………………………….. [3]

4 Anglo-Chinese Junior College 2026 J2 H2 9477 Paper 2 Preliminary Examination For Examiner Use (c) The rate at which plants use photosynthesis to transfer light energy into chemical energy that is stored in plant biomass can be measured in megajoules per square metre per year (MJ m–2 yr –1). Figure 1.2 compares the rates of energy transfer for different ecosystems. Figure 1.2 With reference to Figure 1.2, identify one limiting factor affecting photosynthesis in the snow forest ecosystem. Explain your answer with relevant data. …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... ……………………………………………………………………………………………………….. [3] [Total: 11]

5 [Turn over Anglo-Chinese Junior College 2026 J2 H2 9477 Paper 2 Preliminary Examination For Examiner Use 2 Carbohydrates, proteins and nucleic acids are types of biological molecules. Within each type, there are examples of molecules that can be described as polymers. (a) Complete Table 2.1 to produce a summary of some named biological polymers. Table 2.1 polymer constituent monomer type of bond between monomers cellulose glucose glycogen glucose collagen peptide DNA DNA nucleotide [2] (b) Explain why a cellulose molecule and a glycogen molecule have very different structures, even though they both have glucose as the constituent monomer. …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... …………………………………………………………………………………………......................... ……………………………………………………………………………………………………….. [3]

6 Anglo-Chinese Junior College 2026 J2 H2 9477 Paper 2 Preliminary Examination For Examiner Use (c) During exercise, glycogen in muscle cells is broken down to glucose, which then undergoes glycolysis. (i) Outline what happens to one molecule of glucose during glycolysis. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………............ [2] (ii) Explain how products of glycolysis can lead to ATP production under aerobic conditions. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………................. …………………………………………………………………………………………............ [4] [Total: 11]

7 [Turn over Anglo-Chinese Junior College 2026 J2 H2 9477 Paper 2 Preliminary Examination For Examiner Use BLANK PAGE

8 Anglo-Chinese Junior College 2026 J2 H2 9477 Paper 2 Preliminary Examination For Examiner Use 3 Neutrase® is an enzyme that is used to hydrolyse proteins in solution. When the enzyme is mixed with a 2% protein solution, the reaction mixture changes from white to colourless. A student carried out an experiment to investigate the effect of copper sulfate and potassium sulfate on the activity of Neutrase®. The student made four reaction mixtures in test -tubes A to D. Test-tubes A to C contained equal volumes of protein solution and 0.1 cm³ of solutions of copper sulfate or potassium sulfate. Test - tube D contained the same volume of protein solution and 0.1 cm³ of distilled water. 0.5 cm³ of a 1% Neutrase® solution was added to test -tube A and immediately placed into a colorimeter. The colorimeter was used to measure