2026 EJC JC2 H2 Biology Prelims P1 (A)

©EJC 2026 9477/01/J2H2PRELIM/2026 [Turn over EUNOIA JUNIOR COLLEGE JC2 Preliminary Examination 2026 General Certificate of Education Advanced Level Higher 2 H2 Biology Paper 1 Multiple Choice 9477/01 21 September 2026 1 hour Additional Materials: Multiple Choice Answer Sheet READ THESE INSTRUCTIONS FIRST Write in soft pencil. Do not use paper clips, glue or correction fluid/tape. Write your name, civics group and registration number on the Answer Sheet in the spaces provided. There are thirty questions on this paper. Answer all questions. For each question there are four possible answers A, B, C and D. Choose the one you consider correct and record your choice in soft pencil on the separate Answer Sheet. Read the instructions on the Answer Sheet very carefully. Each correct answer will score one mark. A mark will not be deducted for a wrong answer. Any rough working should be done in this booklet. The use of an approved scientific calculator is expected, where appropriate. This document consists of 23 printed pages and 1 blank page.

2 ©EJC 2026 9477/01/J2H2PRELIM/2026 Answer all questions. 1 Which statement correctly explains the result when the two molecules shown in the diagram react together? A A hydrolysis reaction is forming the disaccharide maltose. B A reducing sugar is split into two monosaccharides by hydrolysis. C Condensation of two α-glucose molecules is taking place. D A condensation reaction is forming two reducing sugars.

3 ©EJC 2026 9477/01/J2H2PRELIM/2026 [Turn over 2 The diagram shows a triglyceride molecule. Which statement is correct for the structure and function of this triglyceride? A The glycerol head forms hydrogen bonds with water in cytoplasm to produce oil droplets. B The hydrophobic fatty acids allow only small molecules to pass through the cell surface membrane. C It packs closely with identical triglyceride molecules to form energy reserves in lysosomes. D It has long hydrocarbon chains that release high energy output per unit mass when oxidised.

4 ©EJC 2026 9477/01/J2H2PRELIM/2026 3 A molecule of haemoglobin is made up of two α polypeptide subunits (α 1 and α 2) and two β polypeptide subunits (β1 and β2). The relative positions of these subunits change when a deoxygenated haemoglobin molecule takes up the oxygen. The axis joining the α2 and β2 subunits rotate by about 15° in relation to the axis joining the α1 and β1 subunits, as shown in the diagram. Which statements about this rotation are correct? 1 The points of contact between the four subunits are altered. 2 The rotation resulting from adding one oxygen molecule to one of the subunits makes it easier to add oxygen to the other subunits. 3 The rotation makes different amino acids available for binding oxygen. 4 The rotation alters the quaternary structure of the molecule A 1, 2 and 3 B 1, 2 and 4 C 1, 3 and 4 D 2, 3 and 4

5 ©EJC 2026 9477/01/J2H2PRELIM/2026 [Turn over 4 The following graphs show the activities of five (1-5) enzymes under different conditions. Which statement is a correct explanation for the rate of reaction of different enzymes? A Kinetic energy of enzyme 3 and its substrates is highest at 75°C. B At pH 2, most of the R groups at the active site of enzyme 4 are all negatively charged. C At pH 8, substrate is bonded to the active site of enzyme 5 by hydrogen bonds only. D At 60°C, many hydrogen bonds between R groups of enzyme 2 are broken.

6 ©EJC 2026 9477/01/J2H2PRELIM/2026 5 The electron micrograph below shows a eukaryotic cell. Which of the following statements about structures P, Q, R and S are correct? 1 S is the site of pyruvate formation. 2 Both P and Q contain templates for transcription. 3 Q contains ribosomal RNA, which is involved in peptide bond synthesis. 4 R has a fluid mosaic structure and regulates the movement of substances between cells. A 1 and 3 only B 2 and 4 only C 1, 2 and 3 only D 1, 2, 3 and 4 S P Q R

7 ©EJC 2026 9477/01/J2H2PRELIM/2026 [Turn over 6 The photomicrograph below shows a plant cell. The cell is 16 eyepiece graticule divisions in width from X to Y. Each eyepiece graticule division is known to be 2.5 µm in length. Which of the following is the closest to the correct magnification of the photomicrograph? A 40 X B 145 X C 400 X D 1450 X

8 ©EJC 2026 9477/01/J2H2PRELIM/2026 7 The graph below shows the relationship between the size, lipid solubility and ability of molecules to cross the cell surface membrane of a mammalian cell. The diameter of the black circles in the diagram is proportional to the size of the molecules. Which of the following could molecules W to Z represent? W X Y Z A glucose water carbon dioxide cholesterol B glucose methane carbon dioxide calcium chloride C calcium chloride methane cholesterol glucose D calcium chloride water glucose cholesterol 8 A new and unusual life form has just been recovered from a meteorite from outer space. It has a genetic code much like those of organisms on Earth except that there are six different DNA bases which were represented by the letters E, U, N, O, I, A. The base sequences are translated as doublets. What is the maximum number of different amino acids that can be accommodated by this genetic code? A 6 B 12 C 36 D 64

9 ©EJC 2026 9477/01/J2H2PRELIM/2026 [Turn over 9 The diagram below represents some biochemical reactions involved in protein synthesis. Which of the following rows is correct? entire molecule coded directly from DNA is represented by 5’ end of molecule enzyme involved in catalysing bond 5 A 1 and 2 Z peptidyl transferase B 1 and 2 Y aminoacyl tRNA synthetase C 1, 2 and 3 X aminoacyl tRNA synthetase D 1, 2 and 4 W peptidyl transferase W X Y Z

10 ©EJC 2026 9477/01/J2H2PRELIM/2026 10 The following table shows the mRNA codons for six different amino acids. mRNA codons amino acid AAA AAG lysine AGA AGG CGG arginine GGU GGA GGC GGG glycine CCU CCA CCC CCG proline UGG tryptophan UAU UAC tyrosine The base sequence of mRNA coding for part of a polypeptide is shown below. U A U A A G A G G C C U U G G 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 start reading From the information provided, which of the predictions stated below is not true? A The insertion of a nucleotide between positions 3 and 4 is expected to result in a greater change in the amino acid sequence than an insertion between positions 12 and 13. B The deletion of a nucleotide at position 5 would result only in an alteration of the second amino acid in the chain. C The substitution of a different nucleotide at position 12 would produce no alteration in the amino acid chain. D The substitution of a different nucleotide at position 13 would result in the alteration of one amino acid.